An Efficient Agrobacterium-Mediated Genetic Transformation Method for a Monocot Model Plant, Brachypodium distachyon

Development of an Efficient Agrobacterium-mediated Transformation Method in Brachypodium Distachyon for the Characterization of Lob Transcription Factors 10

Identification and Characterization of NF-Y Transcription Factor Families in the Monocot Model Plant Brachypodium distachyon

BACKGROUND Nuclear Factor Y (NF-Y) is a heterotrimeric transcription factor composed of NF-YA, NF-YB and NF-YC proteins. Using the dicot plant model system Arabidopsis thaliana (Arabidopsis), NF-Y were previously shown to control a variety of agronomically important traits, including drought tolerance, flowering time, and seed development. The aim of the current research was to identify and cha...

Genetic transformation of the medicinal plant Ruta graveolens L. by an Agrobacterium tumefaciens-mediated method

Ruta graveolens L. is a source of pharmacologically active molecules such as furocoumarins, furoquinolines and acridone alkaloids. A genetic transformation method using Agrobacterium tumefaciens was developed for this plant species. The conditions for an efficient regeneration of R. graveolens led us to retain hypocotyls among other explants tested. Subsequently, transformation was obtained by ...

Use of Agrobacterium rhizogenes Strain 18r12v and Paromomycin Selection for Transformation of Brachypodium distachyon and Brachypodium sylvaticum

The genetic transformation of monocot grasses is a resource intensive process, the quality and efficiency of which is dependent in part upon the method of DNA introduction, as well as the ability to effectively separate transformed from wildtype tissue. Agrobacterium-mediated transformation of Brachypodium has relied mainly on Agrobacterium tumefaciens strain AGL1. Currently the antibiotic hygr...

Plant proteins involved in Agrobacterium-mediated genetic transformation.

Agrobacterium species genetically transform plants by transferring a region of plasmid DNA, T-DNA, into host plant cells. The bacteria also transfer several virulence effector proteins. T-DNA and virulence proteins presumably form T-complexes within the plant cell. Super-T-complexes likely also form by interaction of plant-encoded proteins with T-complexes. These protein-nucleic acid complexes ...